In Vitro Evolution Predicts that the IMP-1 Metallo- -Lactamase Does Not Have the Potential To Evolve Increased Activity against Imipenem
نویسنده
چکیده
In vitro evolution was used to predict whether the IMP-1 metallo-lactamase has the potential to evolve an increased ability to confer resistance to imipenem. Screening of eight libraries containing 9.8 10 1.4 10 (mean standard error) variants per library, with an average of 1.2 mutations per variant, detected no increased resistance to imipenem. The results predict, with >99.9% confidence, that even under intense selection the IMP-1 -lactamase will not evolve to confer increased resistance to imipenem.
منابع مشابه
In vitro evolution predicts that the IMP-1 metallo-beta-lactamase does not have the potential to evolve increased activity against imipenem.
In vitro evolution was used to predict whether the IMP-1 metallo-beta-lactamase has the potential to evolve an increased ability to confer resistance to imipenem. Screening of eight libraries containing 9.8 x 10(6) +/- 1.4 x 10(6) (mean +/- standard error) variants per library, with an average of 1.2 mutations per variant, detected no increased resistance to imipenem. The results predict, with ...
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